recipient cells (Addgene inc)
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recipient cells
Recipient Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pten+gfp+plasmid/pcDNA3+GFP+GFP+PTEN+(Plasmid+%2320739)/pmc12619356-29-31-34
Average 90 stars, based on 14 article reviews
Recipient Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pten+gfp+plasmid/pcDNA3+GFP+GFP+PTEN+(Plasmid+%2320739)/pmc12619356-29-31-34
Average 90 stars, based on 14 article reviews
recipient cells - by Bioz Stars,
2026-09
90/100 stars
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Plasmid Preparation:Article Title: PTEN-regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression. Article Snippet: Cells were infected with high-titer lentivirus containing the shRNA sequences and selected using puromycin (2 μg/mL). si/shRNA sequences are described in Supplementary Table S2. siRNA transfection Cells at 50–80% confluence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. .. Article Title: A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. Article Snippet: The PIK3CA overexpression plasmid was a gift from Jean Zhao (Addgene plasmid #12522). .. The Article Title: Glioma Cell Growth Inhibition Following Photochemical Internalization Enhanced Non-Viral PTEN Gene Transfection Article Snippet: .. Both pcDNA3-EGFP plasmid (Addgene plasmid 13031, submitted by Dr. Doug Golenbock, University of Massachusetts) and Article Title: PTEN regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression Article Snippet: MAT-LyLu (MLL) rat prostate cancer cells with TetOFF-regulated SSeCKS/Akap12 expression [9] were grown in DMEM/10% FBS supplemented with 8 μg/ml of hygromycin, 1 μg/ml puromycin and 0.7 μg/ml tetracycline. sh/siRNA: The RPCCC Gene Modulation Core provided the PTEN shRNA clones (Dharmacon) V2LHS_192536, V2LHS_92314, and control pGIPZ. .. Cells were infected with high-titer lentivirus containing the shRNA sequences and selected using puromycin (2 μg/mL). si/shRNA sequences are described in Supplementary Table S2. siRNA transfection: Cells at 50–80% con uence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. Plasmids: Article Title: PTEN-regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression Article Snippet: Cells at 50–80% confluence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. .. Article Title: A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells Article Snippet: The PIK3CA overexpression plasmid was a gift from Jean Zhao (Addgene plasmid #12522). .. The Sequencing:Article Title: PTEN-regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression. Article Snippet: Cells were infected with high-titer lentivirus containing the shRNA sequences and selected using puromycin (2 μg/mL). si/shRNA sequences are described in Supplementary Table S2. siRNA transfection Cells at 50–80% confluence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. .. Article Title: PTEN regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression Article Snippet: MAT-LyLu (MLL) rat prostate cancer cells with TetOFF-regulated SSeCKS/Akap12 expression [9] were grown in DMEM/10% FBS supplemented with 8 μg/ml of hygromycin, 1 μg/ml puromycin and 0.7 μg/ml tetracycline. sh/siRNA: The RPCCC Gene Modulation Core provided the PTEN shRNA clones (Dharmacon) V2LHS_192536, V2LHS_92314, and control pGIPZ. .. Cells were infected with high-titer lentivirus containing the shRNA sequences and selected using puromycin (2 μg/mL). si/shRNA sequences are described in Supplementary Table S2. siRNA transfection: Cells at 50–80% con uence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. Plasmids: Article Title: PTEN-regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression Article Snippet: Cells at 50–80% confluence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. .. Western Blot:Article Title: PTEN-regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression. Article Snippet: Cells were infected with high-titer lentivirus containing the shRNA sequences and selected using puromycin (2 μg/mL). si/shRNA sequences are described in Supplementary Table S2. siRNA transfection Cells at 50–80% confluence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. .. Article Title: PTEN-regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression Article Snippet: Cells at 50–80% confluence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. .. Transformation Assay:Article Title: Glioma Cell Growth Inhibition Following Photochemical Internalization Enhanced Non-Viral PTEN Gene Transfection Article Snippet: .. Both pcDNA3-EGFP plasmid (Addgene plasmid 13031, submitted by Dr. Doug Golenbock, University of Massachusetts) and Article Title: PTEN and TRAIL genes loaded zein nanoparticles as potential therapy for hepatocellular carcinoma Article Snippet: Gene therapy is one of the recent approaches in treatment of hepatocellular carcinoma (HCC).. Development of a vector or vehicle that can selectively and efficiently deliver the gene to target cells with minimal toxicity is an urgent demand.. In the present study, Phosphatase and Tensin homolog (PTEN) and Tumor Necrosis Factor (TNF)-Related Apoptosis-Inducing Ligand (TRAIL) genes were loaded to Zein nanoparticles (ZNPs). Infection:Article Title: PTEN regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression Article Snippet: MAT-LyLu (MLL) rat prostate cancer cells with TetOFF-regulated SSeCKS/Akap12 expression [9] were grown in DMEM/10% FBS supplemented with 8 μg/ml of hygromycin, 1 μg/ml puromycin and 0.7 μg/ml tetracycline. sh/siRNA: The RPCCC Gene Modulation Core provided the PTEN shRNA clones (Dharmacon) V2LHS_192536, V2LHS_92314, and control pGIPZ. .. Cells were infected with high-titer lentivirus containing the shRNA sequences and selected using puromycin (2 μg/mL). si/shRNA sequences are described in Supplementary Table S2. siRNA transfection: Cells at 50–80% con uence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. Plasmids: shRNA:Article Title: PTEN regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression Article Snippet: MAT-LyLu (MLL) rat prostate cancer cells with TetOFF-regulated SSeCKS/Akap12 expression [9] were grown in DMEM/10% FBS supplemented with 8 μg/ml of hygromycin, 1 μg/ml puromycin and 0.7 μg/ml tetracycline. sh/siRNA: The RPCCC Gene Modulation Core provided the PTEN shRNA clones (Dharmacon) V2LHS_192536, V2LHS_92314, and control pGIPZ. .. Cells were infected with high-titer lentivirus containing the shRNA sequences and selected using puromycin (2 μg/mL). si/shRNA sequences are described in Supplementary Table S2. siRNA transfection: Cells at 50–80% con uence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. Plasmids: Transfection:Article Title: PTEN regulated PI3K-p110 and AKT isoform plasticity controls metastatic prostate cancer progression Article Snippet: MAT-LyLu (MLL) rat prostate cancer cells with TetOFF-regulated SSeCKS/Akap12 expression [9] were grown in DMEM/10% FBS supplemented with 8 μg/ml of hygromycin, 1 μg/ml puromycin and 0.7 μg/ml tetracycline. sh/siRNA: The RPCCC Gene Modulation Core provided the PTEN shRNA clones (Dharmacon) V2LHS_192536, V2LHS_92314, and control pGIPZ. .. Cells were infected with high-titer lentivirus containing the shRNA sequences and selected using puromycin (2 μg/mL). si/shRNA sequences are described in Supplementary Table S2. siRNA transfection: Cells at 50–80% con uence in 6-well tissue culture plates were transfected with siRNA in Lipofectamine 2000 or 3000 (Invitrogen) as per manufacturer’s protocol, and then cell lysates were analyzed after 48–72 h. Plasmids: |
